serum osteocalcin quidelortho cat# 8001, rrid: ab_3099729  (Quidel)

Journal: Research

Article Title: Imbalanced Skeletal Muscle Mitochondrial Proteostasis Causes Bone Loss

doi: 10.34133/research.0465

Figure Lengend Snippet: Skeletal muscle-specific ablation of LONP1 causes bone loss and mechanical impairments. (A to D) Scheme (A) for proteomic analysis using muscle samples from HU and control mice. Data were obtained from published dataset PXD041190. (B) Heatmap showing differentially regulated proteins in HU muscles ( n = 5). (C) GO analysis from up-regulated proteins in HU muscles ( n = 5). (D) Bar graphs for comparison of normalized protein expression of LONP1 in muscle tissues from HU muscles and controls ( n = 5). (E to N) WT and LONP1 mKO male mice were harvested at 8 weeks old. (E and F) μCT pictures (E) and quantification (F) of trabecular bone parameters in the distal femur metaphysis ( n = 6). (G) Bending test analysis of elastic modulus, maximum force, compressive ultimate stress, and post-yield displacement of femurs ( n = 6). (H and I) μCT pictures (H) and quantification (I) of trabecular bone parameters in the fifth LVs ( n = 6). (J) Pictures of calcein double labeling in femur sections (scale bar, 50 μm) and quantification of MAR ( n = 5). (K) Images of ALP staining in femur sections (scale bar, 50 μm) and quantification of ALP + cell surface per bone surface (ALP + .s/BS) in femurs ( n = 5). (L) TRAP staining pictures in femur sections (scale bar, 50 μm) and quantification of TRAP + cell surface per bone surface (TRAP + .s/BS) in femurs ( n = 5). (M and N) Serum osteocalcin levels (M) and CTX-1 levels (N) ( n = 8). Data are shown as the mean ± SEM. * P < 0.05 versus corresponding controls. P values were determined by an unpaired 2-tailed Student’s t test.

Article Snippet: Serum osteocalcin (Novus,NBP2-68151), CTX-1 (Cloud-Clone, CEA665Mu), and FGF21 (Proteintech, KE10042) in male LONP1 mKO, LONP1 HSA-MCM , MCK-ΔOTC, and LONP1/ATF4 DmKO mice and serum FGF21 (Mlbio, ml058174) in humans were conducted using enzyme-linked immunosorbent assay (ELISA) kits.

Techniques: Control, Muscles, Comparison, Expressing, Labeling, Staining

Journal: Research

Article Title: Imbalanced Skeletal Muscle Mitochondrial Proteostasis Causes Bone Loss

doi: 10.34133/research.0465

Figure Lengend Snippet: The sudden loss of LONP1 in mature muscles results in bone loss. (A) Diagrammatic drawing demonstrating the generation of tamoxifen-inducible muscle-specific deletion of Lonp1 (LONP1 HSA-MCM ). (B to I) LONP1 f/f and LONP1 HSA-MCM male mice were fed CD and injected by tamoxifen at 6 weeks old and harvested at 15 weeks old. (B) Representative quantification of LONP1 protein expression in skeletal muscles, eWAT, liver, and heart from LONP1 f/f and LONP1 HSA-MCM male mice. Quantification of the LONP1/β-actin ratio was determined by ImageJ ( n = 3). (C and D) μCT pictures (C) and quantification (D) of trabecular bone parameters in the distal femur metaphysis ( n = 5). (E) Bending test analysis of elastic modulus, maximum force, compressive ultimate stress, and post-yield displacement of femurs from LONP1 f/f and LONP1 HSA-MCM mice ( n = 5). (F and H) Pictures of ALP staining and TRAP staining in femur sections (scale bar, 50 μm). (G and I) Serum osteocalcin levels (G) and CTX-1 levels (I) of LONP1 f/f and LONP1 HSA-MCM mice ( n = 8). Data are shown as the mean ± SEM. * P < 0.05 versus corresponding controls. P values were determined by an unpaired 2-tailed Student’s t test. Ud, undetectable.

Article Snippet: Serum osteocalcin (Novus,NBP2-68151), CTX-1 (Cloud-Clone, CEA665Mu), and FGF21 (Proteintech, KE10042) in male LONP1 mKO, LONP1 HSA-MCM , MCK-ΔOTC, and LONP1/ATF4 DmKO mice and serum FGF21 (Mlbio, ml058174) in humans were conducted using enzyme-linked immunosorbent assay (ELISA) kits.

Techniques: Muscles, Injection, Expressing, Staining

Journal: Research

Article Title: Imbalanced Skeletal Muscle Mitochondrial Proteostasis Causes Bone Loss

doi: 10.34133/research.0465

Figure Lengend Snippet: Skeletal muscle-specific overexpression of mitochondrial-retained ΔOTC protein induces bone loss. (A to J) NTG and MCK-ΔOTC male mice were harvested at 8 weeks old. (A and B) μCT pictures (A) and quantification (B) of trabecular bone parameters in the distal femur metaphysis ( n = 5). (C) Bending test analysis of elastic modulus, maximum force, compressive ultimate stress, and post-yield displacement of femurs from NTG and MCK-ΔOTC mKO. (D and E) μCT pictures (D) and quantification (E) of trabecular bone parameters in the fifth LVs ( n = 5). (F) Pictures of calcein double labeling in femur sections (scale bar, 50 μm) and quantitative analysis of MAR ( n = 5). (G) Images of ALP staining in femur sections (scale bar, 50 μm) and quantification of ALP + .s/BS ( n = 5). (H) Pictures of TRAP staining in femur sections (scale bar, 50 μm) and quantification of TRAP + .s/BS ( n = 5). (I and J) Serum osteocalcin levels (I) and CTX-1 levels (J) ( n = 8). Data are shown as the mean ± SEM. * P < 0.05 versus corresponding controls. P values were determined by an unpaired 2-tailed Student’s t test.

Article Snippet: Serum osteocalcin (Novus,NBP2-68151), CTX-1 (Cloud-Clone, CEA665Mu), and FGF21 (Proteintech, KE10042) in male LONP1 mKO, LONP1 HSA-MCM , MCK-ΔOTC, and LONP1/ATF4 DmKO mice and serum FGF21 (Mlbio, ml058174) in humans were conducted using enzyme-linked immunosorbent assay (ELISA) kits.

Techniques: Over Expression, Labeling, Staining

Journal: Research

Article Title: Imbalanced Skeletal Muscle Mitochondrial Proteostasis Causes Bone Loss

doi: 10.34133/research.0465

Figure Lengend Snippet: Skeletal muscle mitochondrial proteostasis stress-induced bone loss is independent of ATF4. (A) Heatmap analysis of muscle-UPR mt myokines and Lonp1 or Atf4 genes in indicated mice (GSE192990; n = 2). (B to J) WT, LONP1 mKO, and LONP1/ATF4 DmKO male mice were harvested at 8 weeks old. (B and C) μCT pictures (B) and quantification (C) of trabecular bone parameters in the distal femur metaphysis ( n = 5 to 6). (D) Bending test analysis of elastic modulus, maximum force, compressive ultimate stress, and post-yield displacement of femurs ( n = 5). (E and F) μCT pictures (E) and quantification (F) of trabecular bone parameters in the fifth LVs ( n = 5 to 6). (G) Pictures of ALP staining in femur sections (scale bar, 50 μm) and quantification of ALP + .s/BS ( n = 5). (H) Pictures of TRAP staining in femur sections (scale bar, 50 μm) and quantification of TRAP + .s/BS ( n = 5). (I and J) Serum osteocalcin levels (I) and CTX-1 levels (J) ( n = 8). Data are shown as the mean ± SEM. * P < 0.05 versus corresponding controls. P values were determined by one-way ANOVA.

Article Snippet: Serum osteocalcin (Novus,NBP2-68151), CTX-1 (Cloud-Clone, CEA665Mu), and FGF21 (Proteintech, KE10042) in male LONP1 mKO, LONP1 HSA-MCM , MCK-ΔOTC, and LONP1/ATF4 DmKO mice and serum FGF21 (Mlbio, ml058174) in humans were conducted using enzyme-linked immunosorbent assay (ELISA) kits.

Techniques: Staining

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Adolescent Girls With Type 1 Diabetes Develop Changes in Bone Prior to Evidence of Clinical Neuropathy

doi: 10.1210/clinem/dgae511

Figure Lengend Snippet: Circulating biomarkers of bone turnover are decreased in adolescent girls with T1D. (A) Serum osteocalcin, a measure of osteoblast function. (B) Serum CTX-1, a marker of osteoclast activity. (C) Correlation between osteocalcin and CTX-1 in control participants and those with T1D. Abbreviations: CTX-1, collagen cross-linked C-telopeptide-1; TD1, type 1 diabetes.

Article Snippet: Serum osteocalcin (QuidelOrtho Cat# 8001, RRID: AB_3099729) and type I collagen cross-linked C-telopeptide (CTX-1; Immunodiagnostic Systems Cat# AC-02F1, RRID: AB_2923399) concentration were determined by ELISA as directed by the manufacturer.

Techniques: Marker, Activity Assay, Control

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Adolescent Girls With Type 1 Diabetes Develop Changes in Bone Prior to Evidence of Clinical Neuropathy

doi: 10.1210/clinem/dgae511

Figure Lengend Snippet: HR-pQCT and bone biomarker values for T1D + DPN.

Article Snippet: Serum osteocalcin (QuidelOrtho Cat# 8001, RRID: AB_3099729) and type I collagen cross-linked C-telopeptide (CTX-1; Immunodiagnostic Systems Cat# AC-02F1, RRID: AB_2923399) concentration were determined by ELISA as directed by the manufacturer.

Techniques: Biomarker Discovery

Journal: Frontiers in Endocrinology

Article Title: The challenge of the differential diagnosis between brown tumors and metastases in parathyroid carcinoma: a case report

doi: 10.3389/fendo.2024.1414896

Figure Lengend Snippet: Biochemical parameters at baseline and after surgery.

Article Snippet: Ionized calcium was measured by ion-selective electrode method (Nova 8 calcium analyzer, Nova Biomedical).Plasma PTH was measured by a third-generation assay (DiaSorin LIAISON 1-84 PTH chemiluminescent immunoassay) and serum 25-hydroxyvitamin D (25[OH]D) was measured by a chemiluminescent immunoassay (IDS-iSYS); Bone-specific alkaline phosphatase (BSAP) by immunoenzymatic assay (OCTEIA Ostase BAP; IDS Ltd., Boldon, Tyne & Wear, UK), serum N-MID osteocalcin (IDS Ltd., Boldon, UK), and S-CTX (Nordic Bioscience Diagnostics A/S, Herlev, Denmark) by ELISA.

Techniques: